The film "Blade 2" was filmed in Prague, Czech Republic, using both the city streets and buildings for shooting. 

Filming Locations:

Street

In the Movie

Scene where Blade walks through the night city towards the house where he has a base (deleted scene).

In Reality

The scene was filmed on Českomoravská Street in Prague. The buildings visible in the screenshot are no longer there, and the road has been significantly widened, with the tram line now on a dedicated lane.

Alleyway

In the Movie

Scene where Blade enters an alleyway where a fight with a vampire awaits him.

In Reality

The scene was filmed in an alleyway of an industrial district in Prague.