Film was filmed primarily in New Orleans, Louisiana, making extensive use of the city's distinctive architecture and university campuses to create a believable college setting. The story follows two undercover cops who are sent to a local college to infiltrate a drug ring, after their successful high school mission in the first film. The production team cleverly used real churches in New Orleans to represent the fictional police headquarters, with the sequel's base being a different church from the original film's location. Much of the college campus action was shot across Tulane University and Loyola University, both located in the Uptown area of the city. These real academic environments gave the film an authentic campus feel without needing to build elaborate sets. The comedy leans heavily into self-aware humor about sequels, with the cast delivering strong performances, particularly in scenes set around fraternity life and campus culture. Some additional production elements, including props and wardrobe displays, were housed at Sony Studios in Culver City, California.

Filming locations:

St. James African Methodist Episcopal Church

In the movie

Scene where the officers arrive at the original "21 Jump Street" headquarters building, the same church location used in the first film, before they are redirected across the street to their new base of operations.

In reality

The scene was filmed a historic African Methodist Episcopal church in the Tremé neighborhood of New Orleans, built in the 19th century. It serves as both a place of worship and a recognizable film location, having appeared in both 21 Jump Street and its sequel as the fictional police headquarters building.